Located in Boston, MA and New York, NY

Responsibilities of the Scientist I/II (iPSC):

• Innovate then execute experiments to generate and differentiate iPSCs from non-model mammalian organisms, develop functional assays, assess and interpret experimental workflows.
• Test novel stem cell culture conditions, sort, screen, expand and differentiate iPSCs according to latest protocols and newly developed approaches, particularly as it relates to the trophoblast lineage.
• Work with team members to assess efficiency of workflows through cellular assays and NGS sequencing approaches.
• Work on project milestones.
• Attend lab meetings, prepare reports and presentations on the progress of projects.
• Great organizational skills and excellent laboratory record maintenance.

Requirements of the Scientist I/II (iPSC):

• PhD in biological sciences, stem cell biology, genetics or related field.
• 4+ years hands-on expertise in mammalian cell culture systems and iPSCs.
• Expert knowledge of iPSC derivation, differentiation, culture, propagation, and transfection methodologies.
• Experience in molecular biology (nucleic and protein extraction, gel electrophoresis) and cloning techniques (restriction enzyme-based cloning, Gibson assembly).